Methodical guidelines consider one of the modern and often used diagnostic serological reactions - the method of enzyme immunoassay. Its components are listed and the methods of their preparation are described, as well as the formulation of the reaction and the accounting of its results. Methodical instructions are recommended for conducting laboratory and practical classes in the disciplines "Veterinary Microbiology and Mycology", "Immunology", "Virusology and Biotechnology" for students of the Faculty of Veterinary Medicine and Biotechnology, specialty 36. 05. 01 Veterinary medicine, directions of training 36. 03. 01 Veterinary and sanitary examination.