I am so happy to make this 4th Cultural DNA Workshop after the great success of the previous three workshops in 2015, 2017, and 2019, respectively. Because of the COVID-19 pandemic situation, we cannot hold the workshop offline and use Gather.Town for the workshop. Again, I want to emphasize here that the term ‘Cultural DNA’ came from the perspective of a meme, a socio-cultural analogy to a gene, trying to comprehend the concept of a meme from the various cultural aspects of design and the notion of a genetic algorithm.This time, we decide our workshop’s theme as Cultural Space on Metaverse. Due to the pandemic era, many people are spending more time in virtual space and interacting with each other easily. As a result, spatial research in a virtual environment and behavioral research in space are becoming more important. The aim of this workshop is to bring together the researchers from academia and practitioners to share future direction and prospects for virtual space.