Training manual on the course "Fundamentals of microbiology and biotechnology" for students of the direction of training 04. 03. 01 "Chemistry" profile "Environmental Chemistry, chemical expertise and environmental safety" under the program of applied bachelor's degree is compiled on the basis of the requirements of the state educational standard of higher professional education, taking into account the formation of the competencies provided by the standard , According to the standard. The manual includes theoretical issues of microbiology and practical issues of biotechnology of aeration wastewater treatment with active sludge . The material of the manual is based on modern research methods and can be used by students and graduate students in both educational and research work.